Four replicate sequencing libraries were prepared from 100ng of Universal Human Reference RNA (Invitrogen QS0639) using the
Watchmaker mRNA kit (Watchmaker Genomics 7K0105) with Elevate Long UDI Adapter Kit Set A (Element Biosciences 830-00010).

Libraries were pooled and sequenced using the AVITI 2x75 Cloudbreak Freestyle Low Output kit. 
FASTQ files were generated using Bases2Fastq with adapter trimming.